Top latest Five attune flow cytometer Urban news
Top latest Five attune flow cytometer Urban news
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Knowledge-enriched characterization of cell populations. Morphological details from photographs can incorporate to your richness of flow cytometry details. By way of example, the figure exhibits an or else regular apoptosis assay making use of Annexin V and PI, introducing mobile imaging to characterize cells in Every inhabitants to expose morphologically unique functions. These insights could not have already been obtained from multiplex staining by yourself.
Irrespective of whether you’re a rookie or a professional, take a look at our assets to advance your investigate and expertise in flow cytometry.
Incorporate acoustic focusing technological innovation with higher-parameter Evaluation for versatile spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating which has a large-velocity brightfield digital camera for simultaneous large throughput flow cytometry and high resolution brightfield imaging.
"An image is just not very worthy of a thousand dots, but it truly helps you to see images. It is excellent to determine photographs that correlate with staining. One example is, AnnexinV-optimistic cells which might be morphologically diverse than nutritious cells, or CD14+ monocytes which can be more substantial and even more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a highly skilled user has gated singlets confidently. Right after analyzing the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates greater than 4% aggregates.
Pair your Attune flow cytometer using an autosampler for amplified workflow efficiency, and increase robotic integration for optimum throughput.
Our researchers will design panels for you personally as persistently you require—gratis, no product obtain required.
The Attune NxT cytometer will be the dependable workhorse instrument your lab can depend upon for constant and trustworthy performance. The Attune CytPix product encompasses a significant-pace brightfield digicam and AI-pushed picture Evaluation computer software, with new updates such as equipment Studying tools and an intuitive consumer interface, enabling Improved facts precision, accuracy, and speedier insights into sample biology.
Potentially most importantly, these situations contain cells of Obviously distinct phenotypes which may lead to incorrect conclusions regarding double positive situations (particularly in rare populations).
AI-pushed automatic impression analysis Along with the Attune Cytometric Software program translates function capabilities into distinctive morphology parameters that could be coupled with typical fluorescence and scatter parameters to reveal highly reproducible populace adjustments and unique subsets.
We previously demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Let’s examine just the inhabitants of finest interest, the double optimistic functions, To find out more. We will now use extended morphological parameters (here, circularity and skewness of depth) to even further analyze the characteristics of such populations and refine gating on these situations, increasing knowledge robustness.
Created-in dimensionality reduction equipment improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate predicted populace resolution in lysed total human blood. Consumer can watch situations from each cluster for visual confirmation of cell phenotypes.
Versatile payment program from Agilent Economic Answers assist you to obtain the essential instrumentation you require for fast challenge, or to improve your lab.
Gating on these extended parameters enables you to swiftly here and correctly establish populations of interest and confirm gating tactic with little if any manual critique. The Attune program provides access to state-of-the-art device learning analysis applications inside of an expertly built consumer interface, which makes it extremely convenient to use.
The Attune Xenith cytometer can be a substantial-parameter spectral analyzer, offering both spectral unmixing and traditional flow capabilities for detailed and versatile mobile Evaluation.
Agilent Value Guarantee assures you at the least 10 years of instrument use within the date you buy. Normally, We're going to credit rating you Using the residual value of the procedure towards an upgraded model.
What gave the impression to be a homogeneous inhabitants is in fact a lot more assorted—an Perception that ought to be thought of when interpreting effects.